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rabbit anti-βactin primary antibody pa1-16889  (Thermo Fisher)


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    Structured Review

    Thermo Fisher rabbit anti-βactin primary antibody pa1-16889
    Rabbit Anti βactin Primary Antibody Pa1 16889, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-%CE%B2actin+primary+antibody/anti+%CE%B2+actin++pa1+16889/pm37003505-244-0-6
    Average 90 stars, based on 1 article reviews
    rabbit anti-βactin primary antibody pa1-16889 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Bicinchoninic Acid Protein Assay:

    Article Title: AAV dose-dependent transduction efficiency in retinal ganglion cells and functional efficacy of optogenetic vision restoration.
    Article Snippet: After quantification of total protein with the PierceTM BCA Protein Assay Kit (23225, Thermo Fisher Scientific), the protein lysates were adjusted to 1 μg/μl, mixed with 2x Laemmli sample buffer (S3401, Sigma-Aldrich), and boiled for 10 min. Total protein samples (20 μg) were run on a 10% SDS-PAGE gel in Tris glycine-SDS buffer (LC2675, Thermo Fisher Scientific) for one hour at 100 V. The proteins were then transferred to a nitrocellulose membrane at 4 °C for 2 h. After blocking with 25% ChemiBlocker (MilliporeSigma) in 1x Tris-buffered saline containing 0.1% Tween 20 (TBST) for 1 h at 4 °C, the membrane was immunostained with the rabbit anti-GFP primary antibody (1:1000; A11122, Life Technologies, CA, USA) and rabbit anti-βactin primary antibody (1:1000; PA5-72633, Life Technologies) in TBST overnight at 4 °C.

    SDS Page:

    Article Title: AAV dose-dependent transduction efficiency in retinal ganglion cells and functional efficacy of optogenetic vision restoration.
    Article Snippet: After quantification of total protein with the PierceTM BCA Protein Assay Kit (23225, Thermo Fisher Scientific), the protein lysates were adjusted to 1 μg/μl, mixed with 2x Laemmli sample buffer (S3401, Sigma-Aldrich), and boiled for 10 min. Total protein samples (20 μg) were run on a 10% SDS-PAGE gel in Tris glycine-SDS buffer (LC2675, Thermo Fisher Scientific) for one hour at 100 V. The proteins were then transferred to a nitrocellulose membrane at 4 °C for 2 h. After blocking with 25% ChemiBlocker (MilliporeSigma) in 1x Tris-buffered saline containing 0.1% Tween 20 (TBST) for 1 h at 4 °C, the membrane was immunostained with the rabbit anti-GFP primary antibody (1:1000; A11122, Life Technologies, CA, USA) and rabbit anti-βactin primary antibody (1:1000; PA5-72633, Life Technologies) in TBST overnight at 4 °C.

    Membrane:

    Article Title: AAV dose-dependent transduction efficiency in retinal ganglion cells and functional efficacy of optogenetic vision restoration.
    Article Snippet: After quantification of total protein with the PierceTM BCA Protein Assay Kit (23225, Thermo Fisher Scientific), the protein lysates were adjusted to 1 μg/μl, mixed with 2x Laemmli sample buffer (S3401, Sigma-Aldrich), and boiled for 10 min. Total protein samples (20 μg) were run on a 10% SDS-PAGE gel in Tris glycine-SDS buffer (LC2675, Thermo Fisher Scientific) for one hour at 100 V. The proteins were then transferred to a nitrocellulose membrane at 4 °C for 2 h. After blocking with 25% ChemiBlocker (MilliporeSigma) in 1x Tris-buffered saline containing 0.1% Tween 20 (TBST) for 1 h at 4 °C, the membrane was immunostained with the rabbit anti-GFP primary antibody (1:1000; A11122, Life Technologies, CA, USA) and rabbit anti-βactin primary antibody (1:1000; PA5-72633, Life Technologies) in TBST overnight at 4 °C.

    Blocking Assay:

    Article Title: AAV dose-dependent transduction efficiency in retinal ganglion cells and functional efficacy of optogenetic vision restoration.
    Article Snippet: After quantification of total protein with the PierceTM BCA Protein Assay Kit (23225, Thermo Fisher Scientific), the protein lysates were adjusted to 1 μg/μl, mixed with 2x Laemmli sample buffer (S3401, Sigma-Aldrich), and boiled for 10 min. Total protein samples (20 μg) were run on a 10% SDS-PAGE gel in Tris glycine-SDS buffer (LC2675, Thermo Fisher Scientific) for one hour at 100 V. The proteins were then transferred to a nitrocellulose membrane at 4 °C for 2 h. After blocking with 25% ChemiBlocker (MilliporeSigma) in 1x Tris-buffered saline containing 0.1% Tween 20 (TBST) for 1 h at 4 °C, the membrane was immunostained with the rabbit anti-GFP primary antibody (1:1000; A11122, Life Technologies, CA, USA) and rabbit anti-βactin primary antibody (1:1000; PA5-72633, Life Technologies) in TBST overnight at 4 °C.

    Saline:

    Article Title: AAV dose-dependent transduction efficiency in retinal ganglion cells and functional efficacy of optogenetic vision restoration.
    Article Snippet: After quantification of total protein with the PierceTM BCA Protein Assay Kit (23225, Thermo Fisher Scientific), the protein lysates were adjusted to 1 μg/μl, mixed with 2x Laemmli sample buffer (S3401, Sigma-Aldrich), and boiled for 10 min. Total protein samples (20 μg) were run on a 10% SDS-PAGE gel in Tris glycine-SDS buffer (LC2675, Thermo Fisher Scientific) for one hour at 100 V. The proteins were then transferred to a nitrocellulose membrane at 4 °C for 2 h. After blocking with 25% ChemiBlocker (MilliporeSigma) in 1x Tris-buffered saline containing 0.1% Tween 20 (TBST) for 1 h at 4 °C, the membrane was immunostained with the rabbit anti-GFP primary antibody (1:1000; A11122, Life Technologies, CA, USA) and rabbit anti-βactin primary antibody (1:1000; PA5-72633, Life Technologies) in TBST overnight at 4 °C.



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